A state-of-the-art Thermo Vanquish UHPLC. Part of the LC-MS instrumentation necessary to perform QC of our library builds (for monitoring chemical reactions and purifying individual samples).
SensiQ Pioneer FE surface plasmon resonance system (for determination of equilibrium dissociation constants).
A fully automated Agilent 1260 Infinity HPLC with a 6-column chiral column array (Chiral Technologies 鈥淚鈥� series). The instrument uses two Agilent 1290 Infinity valve drives to cycle through the columns on full auto (for method scouting) or on single-column demand (for chiral analysis of previously prepared compounds).
Equipment for Medicinal Chemistry and DNA-Encoded Chemistry Technology:
High-resolution Molecular Structure
For drug discovery, structural biology, and metabolomics
- Bruker 800 MHz NMR with cryoprobe and autosampler
- Bruker 600 MHz NMR
Other Instrumentation for Sophisticated Purification and QC
- Five Teledyne CombiFlash Rf* flash chromatography systems and one CombiFlash Rf* Purlon flash chromatography/mass spectrometry system (compound purification)
- Three ION Torrent Personal Genome Machine sequencing platform with Guava easyCyte Flow Cytometer (high-efficiency sample QC)
- Two Thermo Electron Vanquish UHPLC/LTQ-XL mass spectrometers (monitoring chemical reactions and purifying individual samples)
- Gilson PLC-2250 Prep HPLC (library purification and flash chromatography for compound purification)
- Seventeen integrated Eppendorf thermocyclers (performing on-DNA library chemistry)
- Bio-Rad Next-Generation Chromatography system (protein purification)
- Forte Bio Blitz system (real-time, label-free binding assays)
- Applied Biosystems Real-Time QPCR system (quantitation and monitoring of library material prior to, during, and after selection)
- Hamilton walk-up automated liquid handling station (optimizing and standardizing library binding and selection steps)
- Ample ultra-cold freezers, chemical hoods, spectrophotometers, mixers, sonicators, heat blocks, ad related small instrumentation
iLab Core Service Instrumentation
Current instrumentation available through iLab core service:
- SensiQ Pioneer FE surface plasmon resonance system (for determination of equilibrium dissociation constants)
- GE MicroCal Auto iTC200 (automated isothermal titration calorimeter to monitor binding energetics)
- Roche Applied Science LightCycler 480 Real-Time PCR System (used for performing thermal shift assays, a rapid approach to analyze protein stability)
- Agilent PlateLoc Plate Sealer (heat sealing system for 鈥�80 degrees C storage)
Additional current instrumentation available through iLab core service
- Tecan Freedom Evo Liquid Handler [a robust liquid transfer system (75nl鈥�200碌l) for 384-well plates with an integrated plate reader, plate washer, and incubator]
- Infinite M1000 Pro Plate Reader (measurements of absorbance, fluorescence, luminescence, AlphaScreen, and AlphaLISA)
- HydroSpeed Plate Washer
- Thermo Scientific Multidrop Combi Microplate Dispenser (for dispensing from one to eight different reagents simultaneously into a variety of microplates within a matter of seconds)
- HP Digital Titration Dispenser (allows compound dispensing from 13pl鈥�5碌l, thus improving titration time and permitting more precise dose-response curves)
- Liconic STX220 Tissue Culture Incubator (converts the Tecan workstation from semi-automated to automated and allows the workstation to function 24/7)
- FLIPR Tetra Real Time Screening System (simultaneously pipette and read 384 well plates in fluorescent mode. Pipette from 1-25碌l in increments of 0.5碌l)
- V&P Scientific Pin Tools (integrated with the Tecan workstation to permit the transfer of 100渭l to 500渭l)
Other equipment utilized in the Center for Drug Discovery
- Thermo Scientific 1300 Series A2 tissue culture hoods
- Thermo Scientific Forma Steri-Cult CO2 tissue culture incubators
- Ample standard laboratory instrumentation
Additionally, we have a variety of compound libraries available for small-molecule screening.
